فصلنامه تازه های بیوتکنولوژی سلولی مولکولی
پیاپی 39 (تابستان 1399)

Escherichia coli infections in poultry have a word wide spread. Serotypies O1, O2, O8, O35, O78 Escherichia coli is involved in the development of generalized colibacillosis disease. Genes such as F17, Sfa, pap, afa, , fimH , crl , iuc, bla, yja, chu, are considered as an Escherichia coli acuity factoro. the aim of the present study were Phylogentic of Escherichia coli isolates involved in Colibacillosis and cellucitis in broiler chickens in shahrebabak(Kerman Province) by Multiplex PCR Technique 

From 117 samples of Escherichia coli (83 colibacillosis samples and 34 cellulitis samples) were isolated. These samples were examined and confirmed by biochemical methods.

Colibacillosis isolates were belonged to A (54.27%), B1 (7.22%), B2 (6.03%) and D (32.53%) phylogroups. Where as, the isolates from cellulitis cases were belonged to three main phylogroups; A (55.88%), B1 (5.88%) and D (38.24%).

Statistical analysis showed a specific association between the presence of crl virulence gene and Phylogrups of A and D (P<0.05) in colibacillosis isolates.
The results showed that the isolates from both diseases in broiler chickens could be assigned to various phylogenetic groups (mainly A). Also, the virulence genes profile of cellulitis E.coli is completely different from that of colibacillosis in this region

Flax seed is a nutritive grain which can prevent cancers and cardiovascular diseases, due to compounds such as omega 3 fatty acid, dietary fiber, protein and lignan. The aim of this study was to investigate the effect of oil extraction method on extraction efficiency and the amount of phenolic and antioxidant substances.

In this research two kinds of yellow and brown flaxseed were used for extraction of oil by cold press and solvent method (petroleum ether and ethanol) and extraction efficiency and phenolic and antioxidant compounds were compared.

The results showed that oil extraction was not possible with cold pressing method of yellow flax, and only meal was obtained. The yield of oil extraction for yellow and brown flax using ethanol were (6.6 ± 0.55 and 42.3 ± 1.15) and, for petroleum ether was (11.3 ± 0.98 and 63.03 ± 2.5) respectively. The evaluation of total phenolic compounds and the antioxidant effect of extracted solvents and cold press showed that there was a significant difference between the amount of phenolic compounds extracted by cold pressing method compared to the solvent method, as well as ethanol and petroleum ether solvents. The amount of phenolic compounds and antioxidant activity in yellow flax was higher than brown flax. Also, the amount of these compounds in the cold pressing method was higher than that of solvents.

The type of oil extraction method is highly impacts on the yield of extracted oil from flaxseeds. Brown flaxseed has significantly more oil and less phenolic and antioxidants compounds compared to yellow flaxseed.

Hyaluronic acid (HA) is a natural linear polysaccharide with excellent viscoelasticity, high water retention capacity and biocompatibility finding wide-range of application in medicine, drug delivery and cosmetics. Currently, microbial fermentation is used for industrial HA production. The present study reports screening and optimization of HA production by clinical Streptococcus equisimilis group C and G (GCS and GGS) isolates from Iranian patients.

The 11 S.equisimilis strains including 9 GCS and 2 GGS were characterized and primary HA production was quantified by carbazol assay. Accordingly, three strains were selected for further optimization. The effect of pH on HA yields at 12 points ranging from 3.1-7.5 and glucose concentrations ranging from 0.3-15 mg/ml at 11 points at optimized pH were determined for selected strains.

The range of HA concentration among 11 strains was 216.6-729.7 µg/ml. The three higher producer strains including GCS-08, GGS-88 and GGS132 with an average production of 573.0, 579.7 and 729.7 µg/ml, respectively was selected. The HA concentrations at optimized pH of selected strains were (6.5, 744.3 µg/ml), (4.8, 598.9 µg/ml) and (6.8, 1041.8 µg/ml) respectively. Cultivation at 15 mg/ml of glucose led to increasing HA yield by GCS-08:1096.4, GGS-88:1234.3 and GGS-132:1993.6 µg/ml.

The findings showed the significant effect of pH and direct correlation of glucose concentration on HA production that can increase the yield up to 2.73 folds. Identification of high producer strain at pH 4.8 would be an advantage for HA production under unusual condition at industrial scale.

Helicobacter pylori infection is a major cause of gastric cancer in humans. The Helicobacter pylori tagD gene, which encodes the thiol peroxidase enzyme, plays an important role in bacterial colonization in the human stomach wall. Research has shown that the GPR83 gene in gastric cancer increases expression, and the aim of this study was to investigate the expression of the GPR83 gene in AGS cells transfected with the recombinant pFLAG-CMV-3-tagD vector by Real Time PCR.

AGS cells were transfused using lipofactamine solution and plasmid carrying the tagD gene encoding Helicobacter pylori or empty plasmid (control). RNA extraction was then performed from cultured cells and cDNA synthesis was performed, and then the eukaryotic expression of Helicobacter pylori gene tagD in AGS cells was investigated by RT-PCR method. The expression of GPR83 genes was evaluated by Real Time PCR method. It should be noted that the enoxin kit was used to evaluate apoptosis, and finally the expression of each gene was evaluated using SPSS software and t-test Indepent statistical tests.

The findings from gene expression analysis showed that the expression of GPR83 gene in AGS cells treated with tagD increased compared to control cells, but this increase in expression was not statistically significant (P = 0.0888).

Overall, the data obtained from this study showed that GPR83 gene expression is altered in cells treated with the Helicobacter pylori tagD gene and seems to play a role in the expression of Helicobacter pylori

Todays, the demand and application areas of nanoparticles, especially silica nanoparticles have been increased, progressively. Hence, there is always an effort to find new sources for producing nanoparticles with minimal cost and environmental problems. In this study, the horsetail plant which is categorized in the group of plants with high silica content has been used to synthesis silica nanoparticles.

To do this, the ash obtained from this plant after the calcination process and several steps of acid leaching was characterized by different methods. The morphology, chemical composition, and crystal structure of the synthesized silica nanoparticles were investigated by the scanning electron microscopy (SEM), X-ray diffraction (XRD), and X-ray fluorescence (XRF) methods, respectively. Also, the specific surface area of the particles was determined by the BET technique.

The results showed that the synthesized silica powder had a high purity (97.5 %), amorphous structure, an average particle size of about 30 nm, and a porous structure with surface area of about 410 m2/g, and pore diameter of 11.6 nm.

Therefore, the ash obtained from the horsetail plant can be used to produce porous silica nanoparticles with appropriate functional properties.

Among semiconductor nanoparticles, the titanium dioxide nanoparticles (TiO2NPs) are being widely consumed in the nanotechnology industry and medicine. TiO2NPs are synthesized in various ways. The purpose of this study was to investigate the effect of coating in the synthesis of TiO2NPs on their ability to induce structural disorder in DNA.

Ultraviolet spectroscopy, fluorescence emission spectroscopy, circular dichroism (CD) spectroscopy, and zeta potential analysis were used to study the structural effects of DNA after interaction with TiO2NPs with ethylene glycol dispersion matrix, TiO2NPs contains HNO3 stabilizer and uncoated TiO2NPs.

The Ultraviolet spectroscopy, fluorescence quenching and binding constants indicated that TiO2NPs with ethylene glycol dispersion matrix interacted with TiO2NPs with a higher binding constant value (2.2Í105 M-1) compare with two other TiO2NPs (2.3Í104 M-1 for uncoated TiO2NPs and 1.1Í105 M-1 for TiO2NPs contains HNO3 stabilizer). The CD spectra indicated TiO2NPs have significant influence on the DNA base stacking. The zeta potential analysis showed the surface charge of DNA especially after the addition of TiO2NPs with ethylene glycol dispersion matrix (-24.60 mV) was reduced.

This study showed that TiO2NPs with ethylene glycol dispersion matrix interact with DNA more strongly compared with two other TiO2NPs. This study indicated that the type of coating of TiO2NPs affects the DNA interaction and consequently the type of coating of nanoparticles is very important in designing nano-drugs especially for cancer treatment.

Lactobacilli are Gram-positive, catalase-negative, non-spore forming, rod-shaped organisms. So far, more than 90 species of these bacteria have been identified. There are several strains of probiotic Lactobacilli in a wide range of human food products. Identification of these bacteria in traditional dairy products makes it possible to use them in the production of delicious industrial dairy products. The selection of reliable methods for the identification of Lactobacilli is of a particular importance. Biochemical methods are time consuming and ambiguous. Molecular methods are more appropriate and precise and can be a substitute for previous methods. The purpose of this study is the isolation of Lactobacilli from bacterial flora in Bandar Abbas traditional cheese and identification these bacteria by using PCR as an effective tool for the recognizing the isolated strains.

Fifty samples of traditional cheese were collected from Bandar Abbas and strains were obtained by phenotypic methods.16 s rRNA primers were used to identify their molecular identity. Then the PCR product was sent to Poya Gene Gostar for sequencing. Obtained sequences were blasted in NCBI GenBank.

3 strains of Lactobacillus include Lactobacillus murinus, Lactobacillus helviticus and Lactobacillus planatrum were identified.

These three strains improve the quality of dairy products in this area and can be used in products manufactured in the industry.

One of the effective methods for protein engineering, especially for improving the capabilities of industrial strains, is the genome shuffling method. This study is based on biocatalytic engineering techniques and DNA shuffling methods.

Pseudomonas aeruginosa wild strain was prepared under the access number JQ917006.1. Mutant strains were evaluated by DES method and compatibility with diazinon concentration. After the protoplast was prepared, the genome was mutated from the mutant library. Fused protoplasts were evaluated for activity.

The activities related to IR1.G1, IR1.D8, IR1.D4 and IR1.D5 strains are 0.234 U/ml, 0.1 U/ml, 0.098 U/ml and 0.066 U/ml, respectively, and IRL1.F2, IRL1.F3 and IRL1.F1 strains have activities of 0.541 mg/L, 0.523 mg/L and 0.509 mg/L, respectively.

The results of evaluation of the first generation of genome shuffling (first round of protoplast fusion) showed that the shuffled strains that were able to grow in the presence of toxin (3000 ml/L diazinon concentration) had better activity than the obtained strain through both methods (toxin concentration gradient and DES method).